Table 2 Metadata represented in SP-Document
From: Using semantics for representing experimental protocols
| Bibliographic metadata | |||
| sp:title of the protocol | Extraction of total RNA from fresh/frozen tissue (FT) | ||
| sp:author name | “Kim M. Linton”, “Yvonne Hey”, “Sian Dibben”, “Crispin J. Miller”, “Anthony J. Freemont”, “John A. Radford”, | ||
| and “Stuart D. Pepper” | |||
| sp:protocol identifier | DOI:10.2144/000113260 | ||
| Descriptive metadata | |||
| sp:application of the protocol | “Methods comparison for high-resolution transcriptional analysis of archival material on Affymetrix Plus 2.0 and Exon | ||
| 1.0 microarrays” | |||
| sp:provenance of the protocol | “The extraction method (steps 2–21) is taken from the method supplied with TRIzol reagent Invitrogen, Paisley, UK).” | ||
| Metadata about the materials | |||
| sp:specimen name | “tumor tissue” | ||
| sp:reagent name | “TRIzol”, “Chloroform”, “Ethyl alcohol”, “Isopropyl alcohol” | ||
| sp:manufacturer name | “Invitrogen”, “Sigma-Aldrich” | ||
| sp:equipment or supplies name | “Tissue storage container”, “Homogenizer blades”, “Forceps”, “Scalpel”, “Scalpel holder” | ||